Role of a Multidrug Efflux System in Antibiotic Resistance of P. haemolytica
The goal of this project is to determine if a specific series of Mannheimia
haemolytica genes encodes for a multiple drug resistance mechanism involving
an efflux pump. The results of the study will provide new insights into the
mergence of antibiotic resistance among bacterial pathogens.
Sponsor: USDA National Research Initiative Competitive Grants Program
PI: Cyril Clarke
Roles of a Multidrug Efflux System and Lipopolysaccharide in Antimicrobial
Resistance of Pasteurella haemolytica
This project is designed to evaluate the contribution of a putative multiple
drug resistance efflux system and lipopolysaccharide composition to survival
of bacteria in vivo, in the absence and presence of antibiotic treatment. These
experiments, conducted in vitro and in vivo, are expected to promote development
of a better understanding regarding the effects of feed antibiotics on animal
and human health.
Sponsor: Oklahoma Agricultural Experiment Station (TRIP)
PI: Cyril Clarke
Upgrade of High-performance Liquid Chromatography System
This project supports acquisition of technology necessary for sensitive and
accurate assay of pharmaceutical agents. Such technology is essential for conducting
pharmacokinetic studies, particularly pharmaceutical contract research.
Sponsor: College of Veterinary Medicine
PI: Cyril Clarke
Regulation of Sperm Exocytosis
The acrosome of a sperm must undergo exocytosis before the sperm can fertilize
an egg. The long-term goal of this project is to understand how acrosomal exocytosis
is controlled in order to gain more control of male fertility. The role of sperm
lipids (desmosterol, cholesterol, and sphingomyelin) and intracellular pH in
this process will be determined.
Sponsor: National Institutes of Health
PI: Nicholas L. Cross
Pathogenic Mechanisms of Excerise-induced Asthma
The focus of this project is to determine the effects of strenuous exercise
on health, particularly when the exercise occurs under adverse environmental
conditions. Specifically of interest is the development of exercise-induced
asthma and the role of exercise-induced oxidative stress on injury and disease
Sponsors: The Oklahoma Center for the Advancement of Science and Technology
(OCAST), the Thoroughbred Charities of America, the International Sled Dog Veterinary
Medical Association, and the Iditarod Trail Committee.
PI: Michael S. Davis
Molecular Mechanisms of Lung Surfactant Secretion
Lung surfactant is a surface-active material that stabilizes alveoli. It is
synthesized and secreted by lung epithelial type II cells. The long-term objective
of this proposed project is to elucidate the molecular mechanisms of lung surfactant
secretion from type II cells including transport of secretory granules to and
fusion with the plasma membrane. Deficiency of lung surfactant is the cause
of respiratory distress syndrome in premature infants. Accomplishing the goals
of this proposal may give a valuable insight to the therapy of pulmonary diseases
such as neonatal respiratory distress syndrome.
Sponsor: National Institutes of Health
PI: Lin Liu
Fluorescence Sequence Detection System to Enhance Animal Health Research
The objectives are to obtain instrumentation and infrastructure for development
and use of real-time, quantitative polymerase chain reaction capability. Applications
of the technology include diagnostics, gene expression studies, and genomic
analysis.
Sponsor: United States Department of Agriculture
PI: Jerry R. Malayer
Expression Library Immunization via Phage Peptide Display
Objectives are to establish methods for whole-genome screening of pathogenic
microorganisms in order to more rapidly and inexpensively generate candidate
peptides for use as vaccines.
Sponsor: Oklahoma Center for the Advancement of Science and Technology
PI: Jerry R. Malayer
Isolation of Unique Gene Sequences from Cytotoxic Clostridium piliforme
The objective is to apply methods of genomic analysis and evaluation to the
understanding of phenotype variation in strains of pathogenic microorganisms.
Sponsor: Oklahoma Agricultural Experiment Station
PI: Jerry R. Malayer
Estrogen-induced Alterations of Gene Expression in the Bovine Uterus
Objectives include the isolation of estrogen- and retinoic acid-responsive gene
products in cattle from the developing fetal reproductive tract, adult female
reproductive tract, and preimplantation embryo.
Sponsor: Oklahoma Agricultural Experiment Station
PI: Jerry R. Malayer
Applied Genomics in Animal Models via Somatic Cell Nuclear Transfer Technology
The objective is the development of instrumentation and infrastructure to generate
transgenic bovine embryos. These embryos will then be used in studies on the
role of specific gene expression in preimplantation embryo growth and development
and the establishment and maintenance of pregnancy.
Sponsor: Oklahoma Agricultural Experiment Station
PI: Jerry R. Malayer
Enhancement of Research Opportunities for Veterinary Students at Oklahoma
State University
The objective is to facilitate and enhance the participation of veterinary students
in animal health-related research activities within the College of Veterinary
Medicine.
Sponsor: Merck-Merial Animal Health Foundation
PI: Jerry R. Malayer
Electron Microscopy Laboratory
The Electron Microscopy Laboratory provides instruction in electron microscopy
at the graduate level and offers a wide-range of microscopy services including
sample preparation, ultra-thin sectioning, transmission and scanning electron
microscopy, and X-ray microanalysis. Researchers may have all of the work done
by lab personnel or do some or all of it themselves using lab equipment.
Sponsor: Oklahoma State University
PIs: Charlotte L. Ownby, Phoebe Doss, Terry Colberg
Canine Model of Muscular Dystrophy
Muscular dystrophy, an inherited disease in humans, is poorly understood and
effective treatments are lacking. Using the canine model of Duchenne muscular
dystrophy, the study is investigating the developmental expression pattern of
the main protein involved in this disease with the goal of developing an effective
treatment in humans.
Sponsor: Oklahoma Center for the Advancement of Science and Technology
PIs: Charlotte L. Ownby, Jerry R. Malayer
Veterinary Pathobiology: Gayman Helman
Veterinary Clinical Sciences: Susan Eddlestone
Physiological Sciences: Karen Vargas
Acquisition of Confocal Laser Scanning Microscope
Confocal laser scanning microscopy is the one of the latest advances in cell
biology that is widely used in research and training of graduate students. This
microscope enables researchers to obtain high-resolution images of labeled molecules
within samples and to observe real time images of dynamic processes in animal
or plant cells.
Sponsor: National Science Foundation
PIs: Charlotte L. Ownby
Entomology and Plant Pathology: Jeanmarie Verchot
Chemistry: Warren Ford
Age and Interactive Toxicity of Organophosphate Insecticides
The project evaluates mechanisms contributing to age-related differences in
sensitivity to organophosphorus insecticides (chlorpyrifos, parathion, and methyl
parathion) and interactive toxicity among those three agents in neonatal, juvenile,
and adult animals.
Sponsor: United States Environmental Protection Agency
PIs: Carey Pope, Jing Liu
Presynaptic Modulation of Anticholinesterase Toxicity
The project evaluates contribution of presynaptic neurochemical mechanisms (e.g.,
neurotransmitter synthesis and release and their modulation) in differential
toxicity of organophosphorus insecticides through maturation and aging.
Sponsor: National Institute of Environmental Health Sciences
PI: Carey Pope
Stress, Organophosphates, and Blood Brain Barrier Permeability
The project evaluates the ability of physical and chemical stressors to modify
blood brain barrier permeability and the neurotoxicity of anticholinesterases.
Sponsor: United States Army
PIs: Carey Pope
Johns Hopkins University: Joseph Bressler
LSU Health Sciences Center: Steve Pruett
Lasalocid-induced Peripheral Neuropathy in Rodents: A Potential Biomedical
Model for Human Peripheral Nerve Disorders
The major goal of this project is to induce and characterize peripheral neuropathy
with Lasalocid, a commonly used ionophore as a coccidiostat and growth promotant
in broiler chicks. If successful, this could serve as potential model for human
peripheral nerve disorders including peripheral neuropathies –induced by
Diabetes Mellitus, anticancer drugs, and AIDS.
Sponsor: College of Veterinary Medicine
PIs: Subbiah Sangiah, Carey Pope
Veterinary Pathobiology: Stanley Van Hooser
Pathophysiologic Role of Endothelin-1 (ET-1) in Ascites Syndrome (AS) in
Broiler Chicks
Ascites syndrome is a primary cause of death for rapidly growing broiler chickens
in many regions of the world, resulting in severe economic loss to the poultry
industries. The major objective of this project is to determine the pathophysiologic
role of ET-1 , a potent vasoconstrictor peptide in pulmonary hypertensive syndrome,
right heart failure, and AS leading to death.
Sponsor: Oklahoma State Department of Agriculture
PIs: Subbiah Sangiah
Veterinary Pathobiology: Stanley Vanhooser
Animal Science: Robert Teeter, Jr.